A radioimmunoassay for 1,25-dihydroxycholecalciferol.

نویسندگان

  • T L Clemens
  • G N Hendy
  • R F Graham
  • E G Baggiolini
  • M R Uskokovic
  • J L O'Riordan
چکیده

1. 1alpha,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1alpha,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1alpha-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1alpha,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1alpha,25-dihydroxycholecalciferol.

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عنوان ژورنال:
  • Clinical science and molecular medicine

دوره 54 3  شماره 

صفحات  -

تاریخ انتشار 1978